First report of ?Candidatus Phytoplasma mali? in Prunus avium, P. armeniaca and P. domestica

'Candidatus Phytoplasma mali', 'Candidatus Phytoplasma pyri' and 'Candidatus Phytoplasma prunorum', the causal agents of apple proliferation, pear decline and European stone fruit yellows, respectively.

Apple proliferation (AP), pear decline (PD) and European stone fruit yellows (ESFY) are among the most economically important plant diseases that are caused by phytoplasmas. Phylogenetic analyses revealed that the 16S rDNA sequences of strains of each of these pathogens were identical or nearly identical. Differences between the three phytoplasmas ranged from 1.0 to 1.5% of nucleotide positions...

Formulation and Evaluation of Sustained Release Tablets Using Prunus armeniaca (L.) and Prunus domestica (L.) Gums

EvaGreen real-time PCR protocol for specific 'Candidatus Phytoplasma mali' detection and quantification in insects.

In this paper the validation and implementation of a Real-time PCR protocol based on ribosomal protein genes has been carried out for sensitive and specific quantification of 'Candidatus (Ca.) Phytoplasma mali' (apple proliferation phytoplasma, APP) in insects. The method combines the use of EvaGreen(®) dye as chemistry detection system and the specific primer pair rpAP15f-mod/rpAP15r3, which a...

‘Candidatus Phytoplasma mali’: the current situation of insect vectors in northwestern Italy

The two acknowledged vectors of ‘Candidatus Phytoplasma mali’ in north-western Italy, the psyllid Cacopsylla melanoneura and the leafhopper Fieberiella florii, have been compared to highlight their role in the apple proliferation epidemiology in relation to their infectivity, transmission efficiency and permanence in apple orchards and in wild vegetation, predominantly hawthorn formations. The ...

Hepatoprotective Activity of Fruits of “prunus Domestica” Manoj Soni*, P. K. Mohanty* and Y.a. Jaliwala

Extract of Methanol:ethanol (70:30) of Prunus domestica was prepared and tested for its hepatoprotective effect against Paracetamol and CCl4 induced hepatitis in rats . Alteration in the levels of biochemical markers of hepatic damage like SGPT, SGOT, ALP,Total bilirubin, direct bilirubin and tissue LPO, GSH, catalase and SOD were tested in both treated and untreated groups. Paracetamol (2 g/kg...